The Donald Danforth Plant Science Center's Integrated Microscopy Facility furnishes up-to-date instrumentation for cellular imaging - we encourage you to contact us for your imaging needs.
The facility’s premiere instrument for live cell imaging is a Leica SP8-X confocal microscope
equipped with white light laser, resonance scanner and HyD detectors. In addition to this we have other fluorescence microscopes
to meet our users' needs.
The facility has a workstation for confocal image processing, equipped with Bitplane Imaris
(for 3D and 4D image reconstruction, tracking, and colocalization analysis) and SVI’s Huygens
deconvolution software (for improved signal:noise and resolution). The Nyquist calculator
can be used to determine the correct settings on the confocal microscope to meet Nyquist resolution standards.
Confocal microscopy requires a solid background in understanding how to optimally acquire image data and prepare it for publication. To that end, the facility offers comprehensive five day confocal microscopy workshops in January and July.
The facility's electron microscopes include a LEO 912 AB energy-filter transmission electron microscope
(TEM) and a Hitachi TM-1000 tabletop SEM. We have state-of-the-art specimen preparation for TEM: ultra-rapid freezing done using a Bal-Tec high pressure freezer
. We also have the ancillary equipment
required for transmission electron microscopy.
A prep lab and darkroom are included in the suite, as are computers for digital processing of images using Imaris software.
The facility director R.H. Berg has considerable experience in the study of plant cell structure and he serves as a resource for users in designing and implementing their imaging experiments.
Services are provided to any interested party and are designed with convenience in mind for the client. For those wanting to use the instrument themselves, qualifying for the self-service fee schedule, this can be done after training by IMF staff. On the other hand, full service is also available, putting the work in the hand of our experienced staff.