Phenotyping - Danforth Plant Science Center

The Bellwether Foundation Phenotyping Facility

An essential part of plant science is understanding how plants respond to their environment. One way our scientists can measure this is by monitoring a plant’s phenotype, such as leaf size and shape, root structure, growth rate in particular conditions, and more. In our Phenotyping Facility, the first of its kind at an academic research institute in the U.S., our scientists are able to gather an unprecedented amount of data about the plants they are studying. This data is used to develop improved and sustainable crops that are better able to withstand conditions like drought and extreme temperatures.

Phenotyping Equipment & Services

LemnaTec Scanalyzer 3D

The Scanalyzer 3D platform consists of multiple digital imaging chambers connected to a controlled environment Conviron growth house by a conveyor belt system, resulting in a continuous imaging loop. As an automated, high-throughput platform, it enables our scientists to repeatedly image more than 1,000 plants at multiple points throughout time.

Our standard phenotyping services include operation and maintenance of the LemnaTec Scanalyzer system, as well as technical support and troubleshooting.

Other extended services may be offered based on time and labor availability, at an additional charge:

Planting, sampling, harvesting, thinning
Ground-truthing
Image processing support
Raspberry Pi setup and troubleshooting

Conviron Growth House

Controlled environment chamber custom manufactured to house a complex moving-field conveyor belt system for delivery of plants to and from the Scanalyzer 3D imaging chambers. Lighting intensity, temperature and relative humidity are precisely controlled, allowing for flexibility in experimental design.

Daily Imaging

RGB imaging allows visualization and quantification of plant color and structural morphology. Near-infrared (NIR) enables visualization and quantification of plant water-use efficiency.

Precision Watering

Watering and weighing stations are customizable to deliver water and nutrients on a specified schedule by volume or weight.

Software

Danforth Center scientists developed PlantCV, an open-source image-processing platform used to analyze the massive data sets generated in a typical experiment.

PhenoVation CropReporter

Stand-alone multi-spectral imaging chamber for detection and analysis of Photosystem II, chlorophyll, anthocyanin and color.

Photosystem II Imaging

Whole plant, LED-induced, direct fluorescence imaging in dark or light adapted conditions, enabling the calculation of Fv/Fm and many other photosynthetic parameters.

Stress Detection

Multi-spectral imaging at 6 bands, enabling detection and quantification of plant health/stress by calculation of Fv/Fm, chlorophyll and anthocyanin content.