Features

The images and movies shown were produced by our scientists using instrumentation in the Integrated Microscopy Facility at the Danforth Center and illustrate a variety of cellular characteristics and structures. These are copyrighted and you may copy and use the images for educational purposes only.  They are not to be sold or published without the written permission of the Danforth Center.

We would like to acknowledge the support of the National Science Foundation Major Research Instrumentation program as the source of funds to purchase the following instruments used in making these images:   Zeiss LSM 510 Meta NLO confocal/multiphoton microscope,  Leica SP8-X confocal microscope (NSF Grant No. DBI-1337680—acquisition of a Leica SP8-X confocal microscope), Leo 912 AB transmission electron microscope, and a BalTec high pressure freezer--we thank NSF for their generous support.

The cell structure gallery contains images of living cells, most made using a confocal microscope, and electron micrographs (black and white images).  The subject matter is captioned on the images.

The cell analgyphs gallery contains stereo views (requiring a pair of red-cyan glasses to perceive a 3D effect) and their subject is indicated in the file name.  Most of these were made using confocal microscopy but some were made using scanning electron microscopy (SEM) or transmission electron microscopy (TEM).

The videos gallery contains animations of fluorescent protein-labeled structures. #1 is a time lapse video showing the number and  motility (one frame imaged every second) of Golgi stacks in an Arabidoposis leaf cell, made using widefield microscopy.  Videos #2 and 3 illustrate the concept of 3D imaging by confocal microscopy:  #2 is the raw data (250 optical sections of the endoplasmic reticulum in tobacco BY-2 cells) and #3 is a digital reconstruction of this three dimensional data.  Taking this further, video #4 is a two color 3D rendering of confocal data acquired from Arabidopsis leaf cells,  in red are microtubules and in green are chloroplasts whose grana (spots therein) furnish the green signal due to the fluorescence of chlorophyll in their thylakoid membranes. Video #5 is a time lapse video of the alga Volvox,  imaged using chlorophyll fluorescence from its chloroplasts and lightsheet microscopy.  Lightsheet  microscopy sweeps a thin sheet of excitation light through the sample, producing optical sections that are rapidly captured by a sensitive digital camera as it does this.  This approach therefore can rapidly capture images of large volumes such as this Volvox colony,  all the while keeping the dose of potentially damaging excitation radiation to a minimum.  The results shown here are that these nascent Volvox  colonies were able to undergo embryogenesis even though they were imaged for over 14 hours, showing little damage from excitation light.  During this embryogenesis the embryos turn inside-out and spin.