Proteomics and Mass Spectrometry Facility

Donald Danforth Plant Science Center • St. Louis, MO

Contact Info:

Manager Russell Williams, PhD

Pricing:

Available Upon Request

Summary:

The Proteomics & Mass Spectrometry Facility is equipped with state-of-the-art technologies for the detailed study of a wide range of biomolecules including proteins, nucleic acids, carbohydrates, lipids, hormones and specialized metabolites. The PMSF is a 1,500 square foot lab space with adjoining office space for the director and facility staff as well as a computer lab for data processing. The facility offers fast, high quality specialized analytical services including: protein extractions from various tissues, liquid chromatography separation of proteins and other biomolecules; high resolution 1D and 2D gel electrophoresis; gel image analysis; protein in-solution and in-gel digestion for mass spectrometry; high-throughput protein identification; accurate protein molecular weight analysis; protein covalent/non-covalent complex analysis; quantitative proteomic analysis; protein post-translational modification analysis; peptide de novo sequencing amino acid analysis; acidic plant hormone analysis; targeted small molecule separation/structure determination; untargeted metabolomics analysis.

Equipment:

  • -20C and -80C freezers
    • Ancillary equipment
      • Centrifuges
        • Chromatography Systems
          • 1100 HPLC (Agilent)
            • 1D and high-resolution 2D gel electrophoresis systems (BioRad and Amersham Biosciences)
              • 2D-microLC (Eksigent)
                • 2D-Nano UHPLC (Eksigent)
                  • GelDoc EZ (BioRad)
                    • Prominence HPLC system (Shimadzu)
                      • Ultimate 3000 UPLC (2 units) and nanoUPLC (Thermo-Fischer Scientific)
                      • Mass Spectrometers
                        • 5975 GC-MS (Agilent)
                          • 6500 QTRAP LC-MS/MS (SCIEX)
                            • Orbitrap Fusion-Lumos LC MS/MS (Thermo-Fischer Scientific)
                              • Q Extactive Hybrid Quadrupole-Orbitrap LC-MS/MS (Thermo-Fischer Scientific)
                                • TSQ Altis LC-MS/MS (Thermo-Fischer Scientific)
                                • Shaker-incubators
                                  • Walk-in cold room

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