Advanced Bioimaging Laboratory
Donald Danforth Plant Science Center • St. Louis, MO
Contact Info:
Director Kirk Czymmek, PhD
Pricing:
Available Upon Request
Summary:
The Advanced Bioimaging Laboratory is a 1,500 sq/ft suite of powerful imaging and analysis platforms as well as supporting preparation equipment for routine and advanced plant microscopy applications, including confocal, super-resolution, macro microscopy, wide-field fluorescence, dissecting, X-ray tomography and X-ray microscopy, transmission electron microscopy, and scanning electron microscopy. The facility has the following capabilities:
• Generate high-resolution 3D and 4D image data
• Produce large area maps and sophisticated multi-location and analysis routines
• Perform brightfield, phase contrast, DIC, sensitive imaging of a variety of fluorophores including time-lapse fluorescence imaging
• Screen for fluorescent protein expression and white light imaging of plant morphology
• 3D visualization and analysis with deconvolution optical sectioning
• Multiscale 3D X-ray tomography of a wide range of sample sizes, at resolutions down to sub-micrometer
The facility also includes equipment for cryo-preparation, processing frozen samples, fast-freeze substitution, ultrathin sectioning, serial sectioning, microdissection, handling frozen sections to glass slides, and sample preparation.
Equipment:
- Leica SP8-X confocal microscope on an inverted platform
- 405 nm diode laser
- WLL Whight light laser (allows as many as 200 laser lines starting from 470 nm to 750 nm)
- Argon lasers: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
- Detectors: photomultiplier tube (PMT), supersensitive hybrid detectors (HyD), transmitted light detectors (PMT)
- Resonant scanner
- Motorized microscope stage with Super Z-galvo scanning insert for fast and precise Z movement, standard insert for microscopy slides and 3 – 6-cm glass-bottom Petri dishes; 96-well plate stage insert
- Objectives:
- HC PL APO 10x/NA 0.40 DRY
- HC PL APO 20x/NA 0.70 DRY
- HC PL APO 40x/NA 1.10 WATER CS2
- Correction Collar 0.14-0.18
- HC PL APO 63x/NA 1.20
- HC PL APO 100x/ NA 1.44 OIL
- Super-Resolution Zeiss Elyra 7 microscope on an inverted platform
- Lattice SIM2, Apotome SIM2, TIRF, 2D & 3D Single Molecule Localization Microscopy capabilities
- Lasers: 405 nm, 488 nm, 561 nm, 642 nm
- Objectives:
- EC Plan-Neofluar 10x/NA 0.3 M27 DRY
- C-Apochromat 40x/NA 1.2 Water Corr FCS; C-Apochromat 63x/NA 1.2 Water Corr M27
- Plan-Apochromat 40x/NA 1.4 Oil DIC M27
- Plan-Apochromat 63x/NA 1.4 Oil DIC M27
- alpha Plan-Apochromat 100x/ NA 1.46 OIL DIC M27
- DuoLink for parallel imaging on two cameras: 2x pco.edge sCMOS (version 4.2 CL HS) 
- Motorized microscope stage with Z-piezo insert for fast and precise Z movement, inserts suitable for slides 76 x 26 mm, Petri dish 30 – 35 mm in diameter, LabTek II chamber, 55 x 24 mm 
- Zeiss AxioZoom.V16 microscope
- Lumen Dynamics X-Cite XYLIS LED Fluorescence Microscope Light Source; Excitation of commonly used fluorophores such as DAPI, CFP, GFP/FITC, YFP, TRITC/mCherry/Texas Red, Cy5 and Cy7
- Objectives:
- PlanNeoFluar Z 1.0x/0.25
- PlanNeoFluar Z 2.3x/0.57
- Fluorescence filter cubes:
- DAPI Filter set 49 (Ex: G 365; BS FT 395; Em: BP 445/50) 
- eGFP Filter set 38 HE (Ex: BP 470/40; BS FT 495; Em: BP 525/50) 
- YFP Filter set 46 (Ex: BP 500/25; BS FT 515; Em: BP 535/350) 
- CFP Filter set 47 (Ex: BP 436/25; BS FT 455; Em: BP 480/40) 
- dsRed Filter set 43 (Ex: BP 545/25; BS FT 570; Em: BP 605/70) 
- Cy5 Filter set 50 (Ex: BP 640/30; BS FT 660; Em: BP 690/50) 
- Camera: ZEISS Axiocam 512 color – color CCD camera 12 Megapixel resolution @ 4250 x 2838, 3.1 x 3.1 um pixels @ 14 bits/pixel, full resolution live image @ 10 fps, max live image speed 36 fps
- Nikon Ni-E wide-field fluorescence imager with an upright configuration
- 16-bit monochromatic digital camera (Hamamatsu Orca Flash 4.0)
- Color CMOS camera (Nikon Fi-3)
- Z-stepper motor for 3D imaging
- Fluorescence filter cubes:
- DAPI Exc 350/50 Em 460/50 Dichroic 40DCLP
- CFP Exc 437/20x Em 480-40 nm Dichroic T455lp 
- YFP Exc 500/20x Em 535/30 nm Dichroic T515lp 
- EGFP Exc 470/40 Em 525/50 Dichroic 495
- dsRed (TRITC/Cy3) Exc 545/305 Em 620/60 Dichroic T570lp 
- Cy5 Exc 620/60 Em700/75 Dichroic T660lpxr
- Objectives:
- Nikon Plan Apo 10x/0.45 DRY ∞/0.17 WD 4.0
- Nikon Plan Apo 20x/0.75 DRY ∞/0.17 WD 1.0
- Nikon Plan Apo 60x/1.2 WATER WD 031-0.28
- Nikon Plan Apo 60x/1.4 OIL Ph3 DM ∞/0.17 WD 0.21
- Thermo Scientific Talos 120C G2 Transmission Electron Microscope
- The Thermo Scientific Helios 5 Hydra DualBeam plasma focused ion beam Scanning Electron Microscope (PFIB-SEM)
- Leica EM ICE High Pressure Freezer
- Leica CM 1950 Cryostat CryoJane system
- Leica Ultracut UCT Ultramicrotome
- Vibratome 1000 Sectioning System
- Software: ORS Dragonfly, Stereology Analyzer v4.3.3, Zeiss Zen Black SR, Zeiss Zen Blue, SVI Huygens Essential Deconvolution