Features

 

LTQ-Orbitrap Velos Pro with ETD from Thermo-Fisher Scientific with TriVersa NanoMate from Advion or Ultimate 3000 RSLCnano HPLC from Dionex

• ESI Chip of the TriVersa consists of an array of 400 nanoelectrospray emitters with different inner diameters allowing for flow rates of 20 to 300 nL/min.
• Nozzles on the ESI Chip provide a long, stable spray, and in infusion mode, eliminate sample-to-sample carryover.
• TriVersa can be used for Chip-based infusion or LC/MS fraction collection
• TriVersa can be coupled to any conventional HPLC system for MS analysis
• Higher pressure capability of the RSLCnano platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples
• Temperature-controlled column compartment for maintaining exceptional retention time reproducibility
• LTQ-Orbitrap Velos Pro has resolution of 60,000 at m/z 400 at a scan rate of 1Hz
• Mass accuracy of < 3 ppm with external calibration
• Equipped with HCD cell for more efficient quantitation of isobarically labeled peptides
• Equipped with Electron Transfer Dissociation (ETD) to generate complementary information for highly sensitive PTM analyses and de novo sequencing
• Parallel MS and MSn analysis



Q-Exactive from Thermo-Fisher Scientific with TriVersa NanoMate from Advion,  2DLC Ultra nanoLC from Eksigent or 1200 capillaryLC from Agilent

 • ESI Chip of the TriVersa consists of an array of 400 nanoelectrospray emitters with different inner diameters allowing for flow rates of 20 to 300 nL/min.
• Nozzles on the ESI Chip provide a long, stable spray, and in infusion mode, eliminate sample-to-sample carryover.
• TriVersa can be used for Chip-based infusion or LC/MS fraction collection
• TriVersa can be coupled to any conventional HPLC system for MS analysis
• The variety of HPLCs that can be coupled to the Q-Exactive allows for nano-capillary separations of proteins and peptides where sample is limiting to small molecule analysis on standard analytical scale chromatography
• Q-Exactive has resolution of ~50,000 at m/z 400 at a scan rate of 1Hz
• Mass accuracy of < 5 ppm with external calibration
• Equipped with HCD cell for more efficient quantitation of isobarically labeled peptides
• Scan range of m/z 50-600 for analysis of small molecules to intact natively folded proteins
• Capable of parallel SIM or MS/MS scans for increased sensitivity and decreased cycle time


6500 QTRAP with SelexION from AB SCIEX with microLC from Eksigent

​• SelexION feature allows resolution of isobaric species using differential mobility spectrometry
• MicroLC can utilize 0.3 to 2 mm ID columns and operate at up to 10,000 PSI
​• Up to 100 fold more sensitive than previous generation LC-MS/MS systems
• Enables high-sensitivity full-scan MS, MS/MS, and MS3 with high-selectivity from true triple   quadrupole precursor ion (PI) and neutral loss (NL) scans.
• Perform multiple reaction monitoring (MRM) for quantitation using high-sensitivity triple quadrupole.
• Identify, characterize, and quantitate metabolites quickly and easily.


5975C GC-MS from Agilent

​• Inert ion source-now programmable up to 350˚C

• Triple-Axis HED-EM Detector

 

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ITQ-900 GC-MS/MS/FID from Thermo-Fisher Scientific

​​• Ion-trap mass spectrometer capable of MS/MS for structure verification/elucidation

• FID detector on the same instrument for parallel analyses

 


Trace GC-FID from Thermo-Fisher Scientific

• GC-FID great for quantitative analysis such as FAMEs etc.




 

Protean IEF Cell from BioRad and IPGphor from Amersham

• First dimension isoelectric focusing unit
• Takes different immobilized pH gradient (IPG) strip




Criterion/Criterion Dodeca Cell from BioRad

• Second dimension gel separation unit
• Runs 13.3 cm x 8.7 cm gels
• Twelve gels simultaneously (Dodeca Cell)


 

PROTEAN II XL Cell from BioRad

• Second dimension gel separation unit
• Accomodates up to 2 gels
• Use with 17 cm IPG strips

Epson Expression Scanner

  • AutoFocus optics
  • 1,600 x 3,200 dpi
  • 42-bit color and 16-bit grey
  • 12.2 x 17.2 inch scan area

 

        

Typhoon 9410 imager from Amersham Biosciences

• High sensitivity and resolution
• 5-orders of magnitude dynamic range
• Various imaging modes:*PhosphorImaging*Blue excited fluorescence (457 nm and 488 nm)*Green excited fluorescence 532 nm*Red excite fluorescence 633 nm*Chemiluminescence   



GelPix spot cutting robot from Genetix

• 8-channel excision head, 10 spots per minute
• Captures both visible and fluorescent images
• Cooled CCD camera, 16-bit Tif format
• 15 x 96 well plates, walkway automation




 

MultiProbe II protein digester from PerkinElmer

• 4 pin head
• Automated protein in-gel or in-solution digestion
• 2x 96 well plate
• Zip tipping and spotting


 

Progenesis SameSpots from NonLinear Dynamics

• Analyze single stain and 2D DIGE experiments.
• Optimize analysis using initial automatic image quality assessment and feedback
• Highly advanced and automated image alignment.
• Automatic analysis of the aligned image set and correction of dye related offsets in DIGE
• Automatically create a ranked list of significant spots based on ANOVA p-value, fold-change and q-values for any groups you compare
• Confirm hits using 2D and 3D montage views, expression profiles and spot data tables


 

MASCOT Search Engine from Matrix Science

• A powerful database search engine
• Integrates all the proven methods of database searching
• Peptide Fingerprint, Sequence Query and MS/MS Ion Search
• Total flexibility in chemical/post-translational modifications 

  
 

Scaffold from Proteome Software

• MS/MS analysis & visualization software
• See proteins identified across multiple samples.
• Combine Sequest and Mascot scores for increased confidence.
• Get more detailed and accurate information from your MS/MS experiments



  

Progenesis LCMS

• Advanced proteomics research solution for label-free quantitative analysis.
• Alignment is automated and based on paired feature detection at the LC-MS level.
• Detection uses isotope profiles to confidently build peptides utilizing three dimensional data.
• Quantification of all peptides followed by independent LC-MS/MS identification.
• Quantitative peptide ion analysis is automatically collated with protein information.  


 

HPLC System Gold from Beckman-Coulter

• Temperature Controlled Autosampler
• Diode Array Detector
• UV/VIS Detector
• Fluorescence Detector

   

ACQUITY UPLC System from Waters

• Reliable, high quality chromatographic separations produced up to 10 times faster than current HPLC methods, reducing time and cost per sample.
• Increased efficiency for sharper peaks and better resolution, resulting in better quantitative and qualitative analyses.




  
     

Agilent 3100 OFFGEL Fractionator

• pI-based OFFGEL fractionation with 0.1 – 0.6 pH resolution depending on consumable kit  used
• In-solution fraction recovery for easy transfer to LC/MS Up to 16 samples fractionated at the same time
• µg to mg load capacity

   


 

TissueLyser II