Integrated Microscopy

The Integrated Microscopy Facility has compiled instrumentation for live cell light microscopy and electron microscopy that strives to furnish the best possible cellular imaging for plant scientists, within the institute and regionally. We offer training for those wanting to acquire their own data and full service for those requiring expertise offered by facility staff. Instrumentation for confocal and two photon light microscopy, and transmission electron microscopy (including high pressure freezing), is described in the instrumentation link on this page.

DESCRIPTION | RESEARCH TEAM | PUBLICATIONS | FEES | FACILITY NEWS

Publications

Lu, Yuqing, Li Cong, Hai Wang, Hao Chen, Howard Berg, and Yiji Xia (2011) AtPPR2, an Arabidopsis pentatricopeptide repeat protein, binds to plastid 23S rRNA and plays an important role in the first mitotic division during gametogenesis and in cell proliferation during embryogenesis. Plant Journal 67: 13-25.
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Marc Libault, Manjula Govindarajulu, R. Howard Berg, Yee Tsuey Ong, Kari Puricelli, Christopher G. Taylor, Dong Xu, and Gary Stacey (2011) A Dual-Targeted Soybean Protein Is Involved in Bradyrhizobium japonicum Infection of Soybean Root Hair and Cortical Cells. Molecular Plant Microbe Interactions 24 (9): 1051-1060.
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Michelle Liberton, Jotham R. Austin, II, R. Howard Berg, and Himadri B. Pakrasi (2011) Unique Thylakoid Membrane Architecture of a Unicellular N2-Fixing Cyanobacterium Revealed by Electron Tomography. Plant Physiology 155: 1656-1666.
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Michelle Liberton, Jotham R. Austin, II, R. Howard Berg and Himadri B. Pakrasi1, (2011) Insights into the complex 3-D architecture of thylakoid membranes in the unicellular cyanobacterium Cyanothece sp. ATCC 51142. Plant Signaling & Behavior 6: 1-4.
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Maoyin Li,a Sung Chul Bahn, Liang Guo, William Musgrave, Howard Berg, Ruth Welti, and Xuemin Wanga (2011) Patatin-Related Phospholipase pPLAIIIb-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis. The Plant Cell 23: 1107-1123.
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Marc Libault, Xue-Cheng Zhang, Manjula Govindarajulu, Jing Qiu, Yee T. Ong, Laurent Brechenmacher, R. Howard Berg, Andrea Hurley-Sommer, Christopher G. Taylor, and Gary Stacey (2010) A member of the highly conserved FWL (tomato FW2.2-like) gene family is essential for soybean nodule organogenesis. Plant Journal 62: 852-864.
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Libault, L., Zhang, X., Govindarajulu, M., Qiu, J., Ong, Y., Brechenmacher, L., Berg, R.H., Hurley-Sommer, A., Taylor, C.G., and Stacey, G. (2010). A member of the highly conserved FWL (tomato FW2.2-like) gene family is essential for soybean nodule organogenesis. Plant Journal, 62: 852-864.
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Zhu, H., Li, G.J., Ding, L., Cui, X., Berg, H., Assmann, S.M., and Xia, Y. (2009). Arabidopsis extra large G-protein 2 (XLG2) interacts with the Gbeta subunit of heterotrimeric G protein and functions in disease resistance. Mol Plant 2, 513-525.
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Govindarajulu, M., Kim, S.Y., Libault, M., Berg, R.H., Tanaka, K., Stacey, G., and Taylor, C.G. (2009). GS52 ecto-apyrase plays a critical role during soybean nodulation. Plant Physiol 149, 994-1004.
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Berg, R.H., Fester, T., and Taylor, C.G. (2009). Development of the root-knot nematode feeding cell. In Cell biology of plant nematode parasitism, R.H. Berg and C.G. Taylor, eds (Springer), pp. 115-152.
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Berg, R. Howard and C.G. Taylor (editors). (2009) Cell biology of plant nematode parasitism. Plant Cell Monographs, Volume 15, Springer: Berlin. 273 pages.
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Silvester, W.B., Berg, R.H., Schwintzer, C.R., and Tjepkema, J.D. (2008). Oxygen responses, hemoglobin and the structure and function of vesicles. In Actinorhizal Symbioses, K. Pawlowski and W.H. Newton, eds (Spring), pp. 105-146.
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Fester, T., Berg, R.H., and Taylor, C.G. (2008). An easy method using glutaraldehyde-introduced fluorescence for the microscopic analysis of plant biotrophic interactions. J Microsc 231, 342-348.
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Dietrich, C.R., Han, G., Chen, M., Berg, R.H., Dunn, T.M., and Cahoon, E.B. (2008). Loss-of-function mutations and inducible RNAi suppression of Arabidopsis LCB2 genes reveal the critical role of sphingolipids in gametophytic and sporophytic cell viability. Plant J 54, 284-298.
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Berg, R.H., and Beachy, R.N. (2008). Fluorescent protein applications in plants. In Fluorescent Proteins, Methods in Cell Biology, K. Sullivan, ed (Elsevier Inc), pp. 153-177.
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Jez, J.M., Schachtman, D.P., Berg, R.H., Taylor, C.G., Chen, S., Hicks, L.M., Jaworski, J.G., Smith, T.J., Nielsen, E., and Pikaard, C.S. (2007). Developing a New Interdisciplinary Lab Course for Undergraduate and Graduate Students: Plant Cells and Proteins. Biochem. Molec. Biol. Educ 35, 410-415
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Asurmendi, S., Berg, R.H., Smith, T.J., Bendahmane, M., and Beachy, R.N. (2007). Aggregation of TMV CP plays a role in CP functions and in coat-protein-mediated resistance. Virology 366, 98-106.
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Liberton, M., Howard Berg, R., Heuser, J., Roth, R., and Pakrasi, H.B. (2006). Ultrastructure of the membrane systems in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Protoplasma 227, 129-138.
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Shin, R., Berg, R.H., and Schachtman, D.P. (2005). Reactive oxygen species and root hairs in Arabidopsis root response to nitrogen, phosphorus and potassium deficiency. Plant Cell Physiol 46, 1350-1357.
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Hammes, U.Z., Schachtman, D.P., Berg, R.H., Nielsen, E., Koch, W., McIntyre, L.M., and Taylor, C.G. (2005). Nematode-induced changes of transporter gene expression in Arabidopsis roots. Mol Plant Microbe Interact 18, 1247-1257.
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Ge, X., Dietrich, C., Matsuno, M., Li, G., Berg, H., and Xia, Y. (2005). An Arabidopsis aspartic protease functions as an anti-cell-death component in reproduction and embryogenesis. EMBO Rep 6, 282-288.
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Berg, R.H. (2004). Evaluation of spectral imaging for plant cell analysis. J Microsc 214, 174-181.
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Asurmendi, S., Berg, R.H., Koo, J.C., and Beachy, R.N. (2004). Coat protein regulates formation of replication complexes during tobacco mosaic virus infection. Proc Natl Acad Sci U S A 101, 1415-1420.
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Bendahmane, M., Szecsi, J., Chen, I., Berg, R.H., and Beachy, R.N. (2002). Characterization of mutant tobacco mosaic virus coat protein that interferes with virus cell-to-cell movement. Proc Natl Acad Sci U S A 99, 3645-3650.
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