Integrated Microscopy

The Integrated Microscopy Facility has compiled instrumentation for live cell light microscopy and electron microscopy that strives to furnish the best possible cellular imaging for plant scientists, within the institute and regionally. We offer training for those wanting to acquire their own data and full service for those requiring expertise offered by facility staff. Instrumentation for confocal and two photon light microscopy, and transmission electron microscopy (including high pressure freezing), is described in the instrumentation link on this page.

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Protocols

  • Stereo anaglyphs
  • Leo Aperture Configurations
  • LEO Short Manual
  • Common Counterstains
  • Congo Red-Mitotracker_protocol
  • DDPSC Zeiss LSM 510 manual
  • Dichlorofluorescein acetate ROS Staining
  • High pressure freezing-freeze substitution Protocol
  • Immunofluorescence
  • Immunogold
  • Wax Embedding Protocol
  • Chemical Fix Protocol

  • Lab Links

  • Image J
  • Optical Microscopy Primer
  • Microscopy Society of America
  • Boulder Lab for 3D EM of Cells
  • Jim Haseloff's Fluorescent Protein Resource
  • SWEHSC Digital Ethics Document
  • Zeiss Fluorescent Dye Filter Database
  • American Society for Cell Biology Seminars
  • Cell Imaging at the Carnegie Institute
  • Plants in Motion