Daniel P. Schachtman

Citizenship: U.S.A. and Australian (dual citizen)

Donald Danforth Plant Science Center
975 N Warson Rd
St. Louis, MO  63132
p: 314-587-1421
f: 314-587-1521

Education

Awards

Affiliations

Research Experience

Grants and Fellowships

Fellowship Grants Prior to 1994

• Rotary International Scholarship to study in Australia (1988)

• Australian University PhD Scholarship (1989 - 1991)

• National Science Foundation USA Postdoctoral Fellowship in Plant Biology (1992 - 1994)

Patents

• High-Affinity Potassium Uptake Transporter From Higher Plants (USA patent #5608145-A March 1997; JI Schroeder and DP Schachtman)

• Nucleic Acids Encoding Metal Uptake Transporters and Their Uses (USA patent application #23070-6871PG; JI Schroeder, DM Antosiewicz, DP Schachtman, S Clemens)

Invited Symposium/Plenary Talks at Conferences

October 1991: Fourth International Symposium - Genetic Aspects of Plant Mineral Nutrition Canberra, Australia

July 1995: Tenth International Workshop on Plant Membrane Biology, Regensberg, Germany

August 1996: Gordon Conference on the Cellular Basis of Salinity and Drought, New Hampshire

August 1998: Eleventh International Workshop on Plant Membrane Biology, Technical Workshop - Transporter Expression in Xenopus Oocytes, Cambridge, UK

September 1999: Australian Society of Plant Physiologists 39th Annual Conference- Peter Goldacre Lecture

July 2002: Gordon Conference on the Cellular Basis of Salinity and Drought, Oxford October 2002 Agricultural Plant Stress II APSRC Symposium, Chonnam National University, Kwangju, South Korea

June 2003: 1^st Pan American Plant Membrane Biology Workshop, Cuernavaca, Mexico

January 2004: Plant and Animal Genome Conference, San Diego, CA

March 2004: Society for Experimental Biology, Edinburgh, UK

July 2004: 13^th International Workshop on Plant Membrane Biology, Montpellier France

Invited seminars (1994 - 2000)

June 1994  High-Affinity Potassium Uptake in Higher Plants, Florigene, Melbourne

August 1994  Potassium Uptake in Higher Plants, Department of Botany, The University of Adelaide

November 1994  Forward & Reverse Genetic Approaches to Understanding Sodium Exclusion in Plants,  Department of Genetics, The University of Adelaide

November 1994  Molecular Mechanisms of Potassium Uptake in Higher Plants, Department of Medical Biochemistry, The Flinders University

January 1995 Molecular Biology of Potassium Transport in Plants,  Curtin Conference Structure and Function of Ion Channels, Canberra

April 1995 A Review of the Molecular Biology of Plant and Animal Potassium Channels, Adelaide Ion Channels Symposium, Medical School, University of Adelaide

April 1996 A PESTy Plant Ion Transporter, Adelaide Ion Channels Symposium, University of Adelaide

February 1997 Changing the Structure and Function of a Potassium Transporter, CSIRO Canberra, Workshop on Molecular Mineral Nutrition of Plants

March 1997 Molecular Plant Mineral Nutrition, Ohio State University, Columbus, Ohio

August 1997 Zinc Efficiency in Wheat, GRDC Workshop on Micronutrients and Medic Decline, Waite Campus, Adelaide

January 1998 Functional and Structural Studies on HKT1, a Na+ Energized High Affinity K+ Transporter, Department of Botany, Cambridge, UK

January 1998 Functional and Structural Studies on Two Membrane Transport Proteins in Higher Plants, Department of Plant Physiology, University of Tubingen, Germany

February 1998 Functional and Structural Studies on HKT1, a Na+ Energized High Affinity K+ Transporter, University of London, Wye College, UK

March 1998 The Structure and Function of Two Plant Cation Transporters, Botany Department, University of Melbourne, Melbourne, Australia

September 1998 Molecular Mechanisms of Plant Mineral Nutrition, Department of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia

October 1998 Connections Between K+ Uptake Mechanisms in Plants, Yeast, and Bacteria Department of Horticulture, Viticulture and Oenology, University of Adelaide,  Adelaide, Australia

April 1999 Molecular structure and function of two cation transporters HKT1 and LCT1 expressed in plant roots CSIRO - Plant Industry Horticulture Unit, Adelaide

September 1999 - Plant membrane transporters "downunder" - enhancing mineral nutrition and resistance to abiotic stress, Australian Society of Plant Physiologists 39^th Annual Conference- Peter Goldacre Lecture,  Gold Coast

September 1999 - Using toxic ions to define regions of HKT1 (a K⁺/Na⁺ transporter) that are involved in potassium and sodium transport Australian Society of Plant Physiologists 39^th Annual Conference, Gold Coast

April 2000 - Uptake and transport of toxic ions from soils to whole plants and molecules Utah State University, Logan, Utah, USA

April 2000 Reducing the uptake and transport of toxic ions from saline soils Iowa State University, Ames, Iowa USA

May 2000 Improving the K+ affinity and transport under saline conditions of a K+/Na+ symport (HKT1) ENSA-M/INRA/CNRS, Montepellier, France

January 2001 Manipulating plant ion transporter structure and function to reduce Na⁺ and Cl^- accumulation under saline conditions Donald Danforth Plant Science Center, St. Louis, MO

January 2001 Manipulating plant ion transporter structure and function to reduce Na⁺ and Cl^- accumulation under saline conditions University of Missouri, Columbia, MO

October 2001  The structure and function of a plant cation transporter (HKT1) expressed in roots, University of Illinois, Champagne, Ill.

November 2001 Plant root function under saline conditions, Donald Danforth Plant Science Center Annual Symposium, St. Louis, MO

January 2002 Understanding and increasing zinc uptake in cereals, University of Missouri, Columbia, Department of Agronomy and Plant Science Unit

February 2002 Engineering zinc uptake in cereal root and whole plants, Washington University, St. Louis, Department of Biology

September 2002  Engineering zinc uptake in cereal root and whole plants, Horticulture Department, Purdue University

October 2002 Plant root response to potassium deficiency, Korea  University, Seoul

April 2003 Scientific presentation to general public at the Saint Louis Science Center entitled "Plant Biotechnology"

September 2003 Potassium Sensing by Arabidopsis Roots, 2^nd Midwest Rhizosphere Symposium, St. Louis

October 2003 Scientific presentation to general public at the Saint Louis Science Center entitled "Plant Biotechnology"

December 2003 Nutrient sensing by Arabidopsis roots, Donald Danforth Plant Science Center, St. Louis  

November 2004 Profiling the xylem sap of maize for changes in minerals, organic acids and proteins under drought,  Instituto de Biotecnologia - UNAM, Cuernavaca, Mexico

Training of students and Research staff (1994-2004)

Honours Students (5 between 1995 and 1997)

Postgraduate students (PhD students)

• Zaida Antoniolli (1999)
  Research Topic: Development of molecular methods for mycorrhizal fungal identification

• Greg Natrass (2000)
  Research Topic: Characterisation of a putative cysteine transporter from sheep hair follicle

• Sunita Ramesh (2002)
  Research Topic: Identification and overexpression of the zinc transporters in cereals

Postdoctoral Fellows

• Raman Kumar 1995 - 1996

• Eugene Diatloff 1996 - 1998

• Garry Rosewarne 1997 – 1999

• Weihong Liu 1998 – 2001

• Sung-ju Ahn 2002 - 2004

• Ryoung Shin 2002 – present (KOSEF Fellow)

• Ulrich Hammes 2002 – present (DFG Fellow)

• Jason Goodger 2003 – present

• Victor Asirvatham 2003 – 2004

• Sophie Alvarez 2004 – present

Reviewed manuscripts for:

Australian Journal of Plant Physiology, Journal of Experimental Botany, Physiologia Plantarum, Planta, Plant and Soil, Plant Cell, Plant Cell and Environment, Plant Journal, Plant Physiology, Proceedings of Nat. Acad. Sci. USA, Protoplasma, Nature Biotechnology, New Phytologist, Theoretical and Applied Genetics

Reviewed grants for:

Australian Research Council, U.S. Department of Agriculture Competitive Grants, Department of Energy, USA, National Science Foundation, USA, Biotechnology and Biological Sciences Research Council, UK

Professional Service

• Honorary Secretary - Australian Society of Plant Physiologists (1996-1999)

• Member - Australian Journal of Plant Physiology Advisory Board (1997- 1999)

• Member of the Emerging Leadership Council (ELC) St Louis Science Center (2003, 2004)

• Women in Plant Biology Committee, American Society Plant Biologists 2003-2006

• NSF Plant Genome Program - Review Panel Member for Spring 2004

• Monitoring Editor Plant Physiology 2004 – 2010

• NSF Integrated Plant Biology - Review Panel Member 2005 - 2007

Committee Activity at Danforth Center

• Danforth seminar committee

• Proteomics/mass spec oversight committee

• IT oversight committee

• Co-organizer of Midwest Rhizosphere Symposium

Publications List

41.  Ramesh, S., Choimes, S. and Schachtman, D.P. (2004) Overexpression of an Arabidopsis zinc transporter in Hordeum vulgare increases short-term zinc uptake after zinc deprivation and seed zinc content  Plant Molecular Biology 54:373-385.

40.  Shin, R. and Schachtman, D.P. (2004)  Hydrogen peroxide mediates plant root cell response to nutrient deprivation  Proceedings National Academy Science USA  101:8827-8832.

39.  Ahn, S.J., Shin, R. and Schachtman, D.P. (2004) Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K⁺ uptake Plant Physiology 134:1135-45.

38.  Mpelasoka, B.S., Schachtman, D.P., Treeby, M.T., Thomas, M.R. (2003) A review of potassium nutrition in grapevines with special emphasis on berry accumulation.  Australian Journal of Grape and Wine 9:154-168.

37.  Storey, R., Wyn Jones, R.G.,  Schachtman D.P.,  and Treeby M.T. (2003) Calcium accumulating cells in the meristematic region of grapevine root apices Functional Plant Biology 30: 719 – 727.

36.  Ramesh, S., Shin, R., Eide, D.J. and Schachtman, D.P. (2003) Differential metal selectivity and gene expression of two zinc transporters from rice.  Plant Physiology 133: 126-134.

35.  Schachtman, D.P. and Thomas, M.R. (2003) A rapid method for generating sufficient amounts of uniform genotype-specific material from woody perennials such as grapevine for ion transport studies.  Plant Soil 253: 195-199.

34.  Storey, R. Schachtman, D.P. Thomas, M.R (2003) Root structure and cellular chloride, sodium and potassium distribution in salinized grapevines.  Plant Cell and Environment 26: 789 - 800.

33.  Munns, R., Husain, S., Rivelli, A.R., James, R., Condon, A.G., Lindsay, M., Lagudah, E., Schachtman, D., Hare, R. (2002) Avenues for increasing salt tolerance of crops, and the role of physiologically-based selection traits.  Plant Soil 247: 93-105.

32.  Amtmann, A., Fischer, M., Marsh, E.L., Stefanovic, A., Sanders, D. Schachtman, D.P. (2001) The wheat cDNA LCT1 generates hypersensitivity to sodium in a salt-sensitive yeast strain Plant Physiology 126:1061-1071.

31.  Liu, W., Fairbairn, D.J., Reid, R.J., Schachtman, D.P. (2001) Characterization of two HKT1 homologues from Eucalyptus camaldulensis that display intrinsic osmosensing capability Plant Physiology 127: 283-294.

30.  Schachtman, D.P.  2000  Molecular insights into the structure and function of plant K⁺ transport mechanisms  Biochimica et Biophysica Acta - Reviews on Biomembranes 1465:127-139.

29.  Liu, W., Schachtman, D.P., Zhang, W.  2000 Partial deletion of  a loop region in the high affinity K⁺ transporter HKT1 changes ionic permeability leading to increased salt tolerance  Journal of Biological Chemistry 275: 27924-37932.

28.  Fairbairn D.J., Liu, W., Schachtman D.P., Gomez-Gallego S., Day S. Teasdale R.D. 2000 Characterisation of two distinct HKT1-like potassium transporters from Eucalyptus camaldulensis Plant Molecular Biology, 43:515-525.

27.  Box, S.L., Schachtman D.P. 2000  The effect of low concentrations of sodium on potassium uptake and growth of  wheat. Australian Journal of Plant Physiology 27: 175-182.

26.  Antoniolli Z.I., Schachtman D.P., Ophel-Keller K. and Smith S.E. 2000 Variation in ribosomal DNA internal transcribed spacers sequences in Glomus mosseae and Gigaspora margarita. Mycological Research 104: 708-715.

25.  Schachtman D.P.  and Liu W. 1999 Molecular pieces to the puzzle of the interaction between potassium and sodium uptake in plants. Trends in Plant Science  4: 281 - 287.

24.  Rosewarne G. R., Barker S.J., Smith S.E. Smith F.A., Schachtman D.P. 1999 A Lycopersion esculentum phosphate transporter (LePT1) implicated in P nutrition of VA mycorrhizal plants.  New Phytologist  144: 507-516.

23.  Merry L.J., Graham R.D., Schachtman D.P. 1999  Triticum tauschii  - a potential source of genes for the improvement of zinc efficiency in bread wheat. In: Plant Nutrition - Molecular Biology and Genetics pp. 235-244  (Eds G. Gissel-Nielsen and A. Jensen) Kluwer Academic Publishers.

22.  Dreyer I., Horeau C., Lemaillet G., Zimmermann S., Bush D.R., Rodriguez-Navarro, A., Schachtman D.P., Spalding E.P., Sentenac H., Gaber R.F. 1999  Identification and characterization of plant transporters using heterologous expression systems. Journal of Experimental Botany  50: 1073-1087.

21.  Smith S.E., Rosewarne G., Ayling S.M., Dickson S., Schachtman D.P., Barker S.J., Reid R.J., Smith F.A.  1998   Phosphate transfer between vesicular-arbuscular (VA) mycorrhizal symbionts: insights from confocal microscopy, microphysiology and molecular studies.  In: Phosphorus in plant biology: regulatory roles in molecular, cellular, organismic and ecosystem processes.  (Eds.  J.P. Lynch, J. Derkman)  American Society of Plant Physiology

20.  Schachtman D.P. and Barker S.J.  1999  Molecular approaches for increasing the micronutrient density in edible portions of food crops.  Field Crops Research 60: 81-92.

19.  Schachtman D.P., Reid R.J., Ayling S.  1998 Phosphorus uptake by plants: from soil to cell.  Plant Physiology  116:447-453.

18.  Clemens S., Antosiewicz D.M., Ward J.M., Schachtman D.P., Schroeder J.I. 1998 The plant transporter LCT1 mediates the uptake of cadmium and calcium. Proceedings National Academy Science USA  95:12043-12048.

17.  Diatloff E., Kumar R., Schachtman D.P.  1998  Site directed mutagenesis reduces the Na⁺ affinity of HKT1, a Na⁺ energized high affinity K⁺ transporter.  FEBS Letters 432: 31-36.

16.  Schachtman D.P., Kumar R., Schroeder J.I., Marsh E.L.  1997 Molecular and functional characterization of a novel low-affinity cation transporter (LCT1) in higher plants.  Proceedings National Academy Science USA 94: 11079-11084.

15.  Bleby T.M., Aucote M., Kennett-Smith A.K. Walker G.R., Schachtman D.P.   1997  Seasonal water use characteristics of the tall wheatgrass (Agropyron elongatum) in a saline environment. Plant Cell and Environment. 20:1361-1371.

14.  Shennan C., Grattan S.R.,  May D.M.,  Hillhouse C.J., Schachtman D.P., Wander M., Roberts B.,  Tafoya S., Burau R.G. , Zelinski L.  1995 Long-term feasibility of irrigating processing tomato with saline drainage water in a three-year rotation with cotton.  Journal of Environmental Quality 24: 476-486.

13.  Munns R., Schachtman D.P., Condon A.G.  1995 Significance of time scale in distinguishing genotypic differences in salt tolerance. Australian Journal of Plant Physiology  22: 561-575.

12.  Schachtman D.P., Schroeder J.I.  1994 Structure and transport mechanism of a high-affinity potassium transporter from higher plants.  Nature  370: 655-658.

11.  Munns, R. and Schachtman D.P.  1993  Plant responses to salinity.  In: International Crop Science I.  D.R. Buxton ed., Crop Science Society of America, Madison, Wis. pp. 741 - 745.

10.  Lagudah E., Appels R., McNeil D. and Schachtman D.P. 1993  Exploiting the diploid D genome for wheat improvement. In: Gene Conservation and Exploitation, 20th Stadler Genetics Symposia Series. Colombia, Missouri. pp. 87-107.

09.  Tyerman S.D. and Schachtman D.P.  1992  The role of ion channels in plant nutrition and prospects for their genetic manipulation.  Plant and Soil  146: 137-144.

08.  Schachtman D.P., Schroeder J.I., Lucas W.J., Anderson J.A., Gaber R.F. 1992  Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science  258: 1654 -1658.

07.  Schachtman D.P., Lagudah E.S. and Munns R.  1992  The expression of salt tolerance from Triticum tauschii in hexaploid wheat. Theoretical and Applied Genetics 84: 714-719.

06.  Schachtman D.P. and Munns R.  1992  Sodium accumulation in Triticum  species that differ in salt tolerance.  Australian Journal of Plant Physiology  19: 331-340.

05.  Schachtman D.P., Tyerman S.D. and Terry B.R.  1991  K+/Na+ selectivity of a cation channel in the plasma membrane of root cells is not different in salt-tolerant and salt-sensitive wheat species.  Plant Physiology  97: 598-605.

04.  Schachtman D.P., Munns R. and Whitecross M.I.  1991  Variation in sodium exclusion and salt tolerance in Triticum tauschii. Crop Science  31: 992-997.

03.  Schachtman D.P. and Kelman W.  1991  Potential of Lotus germplasm for the development of salt, aluminum and manganese tolerant pasture plants.  Australian Journal of Agricultural Research  42: 139-140.

02.  Shennan C., Schachtman D.P. and Cramer G.  1990  Variation in [75SE] selenate uptake and partitioning among tomato cultivars and wild species. New Phytologist  115: 523-530.

01.  Schachtman D.P., Bloom A.J. and Dvorak J.  1989  Salt-tolerant Triticum X Lophopyrum derivatives limit the accumulation of sodium and chloride ions under saline-stress.  Plant, Cell and Environment  12: 47-55.