CBN-V Video Archives - S8-12
Serological and Nucleic Acid Based Detection of Indian Cassava Mosaic Disease

Makeshkumar T. and R.R. Nair

Central Tuber Crops Research Institute, Thiruvananthapuram, India
makesh@ctcri.ren.nic.in

        Cassava mosaic disease (CMD) caused by Indian cassava mosaic virus (ICMV), remains a serious problem in India, which causes high yield losses. Early detection of this disease is essential for an effective management. Serological assay of infected cassava plants with a panel of monoclonal antibody of African cassava mosaic virus, East African cassava mosaic virus and ICMV showed positive reactions with only antibodies of ICMV, SCR 58 and SCR 60. This reveals that the CMD in India is caused by ICMV. Serodiagnosis of different types of symptom with the monoclonal antibodies of ICMV showed the order of increasing virus concentration from mild mosaic to severe distortion symptoms and the absence of the virus in meristem derived plants. In nucleic acid spot hybridisation test using ICMV DNA-A as a probe, ICMV were detected from CMD affected plants. Distribution of ICMV in infected plant is also discussed.

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