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CBN-V Video Archives - S8-11
Molecular Cloning of Indian Cassava Mosaic Virus and its Comparison
with Other Whitefly Transmitted Geminiviruses
Makeshkumar T1., V.G. Malathi2, G.
Radhakrishnan2 and S. Edison1
1. Central Tuber Crops Research
Institute, Thiruvananthapuram, India
makesh@ctcri.ren.nic.in
2. Advanced Centre for Plant Virology, Division of Plant Pathology,
IARI, New Delhi
Cassava mosaic disease has emerged as a serious limiting factor of
cassava production in India, which is caused by Indian cassava
mosaic virus (ICMV). It spreads primarily through the
indiscriminate use of infected stem cuttings as planting material
and secondarily through the whitefly Bemisia tabaci vector.
Supercoiled DNA of ICMV was isolated from CMD affected cassava
leaves by CTAB method followed by CsCl density gradient and cloned
in pUC 18 vector. Clones having DNA-A and DNA-B were identified.
Replicase and Coat protein genes of ICMV were amplified and
sequenced. Comparison of conserved region and these genes’ sequences
with those of other cassava mosaic viruses gave further convincing
evidence that ICMV differs from ACMV and EACMV. Relationship of
these genes’ sequences with other important whitefly transmitted
begomoviruses present in India is discussed.
Speaker's PowerPoint Presentation
2007©
Donald Danforth Plant Science Center All rights reserved.
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