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CBN-V Video Archives - S7-38
Genetic Transformation of Cassava Cultivar TMS 60444 with N Rep Gene
Construct from South African Cassava Mosaic Virus
Makwarela M.1,2 , N.J. Taylor1, C.M. Fauquet1
and M.E.C. Rey2
1.
International Laboratory for Tropical Agriculture Biotechnology (ILTAB)/Donald
Danforth Plant Science Center, 9757 North Warson Road, Saint Louis,
MO 63132, USA
iltab@danforthcenter.org
2. School of Molecular and Cell Biology, University of the
Witwatersrand, P/Bag 3, WITS 2050, Johannesburg, South Africa
Recently, there has been an enormous upsurge of interest in cassava
in South Africa (SA) for industrial applications such as the
extraction of starch for animal feed and paper manufacture, and as a
potential cash crop or food security for marginalized farmers. Our
research program has identified the need to genetically improve
local South African cassava cultivars towards resistance against
African cassava mosaic disease (ACMD) caused by South African
cassava mosaic virus (SACMV) and other cassava-infecting
begomoviruses. In this study, we report the construction of a vector
containing the truncated N Rep protein sequenced from SACMV for
cassava transformation and production of target tissue for cassava
transformation via particle bombardment.
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Donald Danforth Plant Science Center All rights reserved.
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