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CBN-V Video Archives - S7-37
Multilocational Field Evaluation of Tissue Culture Derived Cassava (Manihot esculenta) in Cameroon

Zok S., S.A. Eyango

J.P. Johnson Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona Regional Centre, P.M.B. 25 BUEA, Cameroun zoksimon@yahoo.com

        In order to answer many questions raised concerning the adaptability of tissue culture derived cassava (Manihot esculenta) to field conditions involving its growth characteristics and yield potentials, trials were established in three locations using cuttings from the second and third cycles of tissue culture derived plants and ordinary plants of cassava. Throughout the vegetative cycle, no significant differences were observed in the sprouting rate and vigour of plants. On the contrary, the source of cassava cuttings had a marked effect on African Cassava Mosaic Virus (ACMV) disease incidence. Plants derived from tissue culture plants of the third cycle and ordinary plants were the least infected by ACMV at Mbalmayo and La Dibamba likely due to their adaptability. The analysis of the number and weight of tuber/plant at harvest was not influenced by the source of the cuttings. Tissue culture derived and non-tissue culture derived plants yielded similarly from 3.9-4.3 kg/plant. The comparative advantage of using tissue culture as a reliable means of sanitation for large scale and rapid multiplication of available clones is discussed.

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