CBN-V Video Archives - S7-33
Genotypic Response Variation to Micropropagation and Effect of Retardents on in vitro Growth in Cassava

Unnikrishnan M., M.N. Sheela, V. Gayathri, J..R. Nair

Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, 695 017, India Ukrsihna2001@yahoo.com

        Studies conducted with indigenous and exotic cassava germplasm accessions (exotic: 426, indigenous: 430, inbred: 127, triploids: 14, hybrids: 14) showed that indigenous collections showed better response to meristem culture and micropropagation than exotics. Triploids also showed better response, though initial growth was slow. Explants (meristems) collected from setts raised in primary nursery responded better than those directly collected from field. Out of the two osmotic retardants studied, sorbitol was found better than mannitol in inducing slow and healthy growth. Silver nitrate (0.001 M) was found effective in reducing leaf shedding and prolonging the storage period. Activated charcoal (0.1%) helped in preventing root browning in cultures stored for longer period.

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