CBN-V Video Archives - S7-17
Induction of Friable Embryogenic Callus and Plant Regeneration in Colombian Commercial Cultivars

López D.¹ P. Chavarriaga¹, J. Tohme¹ and W.M. Roca²

1. Biotechnology Research Unit, International Center for Tropical Agriculture (CIAT) -A.A. 6713 Cali, Colombia p.chavarriaga@cgiar.org
2. CIP, Apartado 1558, Lima, Peru

        Friable embryogenic callus (FEC) induction was tested in cassava commercial genotypes MTai8, CM3306-4, CM523-7, CM4574-7, CM6740-7, CM7951-7, MBra383, SM909-25, SM1219-9, MCol2215 and TMS60444 (MNig11). FEC was induced from somatic embryos obtained on MS medium plus 2,4-D, transferred to GD medium plus Picloram for 80 days, and subculture every 20 days. FEC was identified in seven of these cultivars. The frequency of FEC production oscillated between 40 and 65.7%, depending on the genotype. Purification and proliferation has been successful with MCol2215, CM3306-4, SM1219-9, and TMS60444. Plants have been regenerated in two of both MCol2215 and TMS60444, and experiments are underway to regenerate plants on the remaining cultivars. It is worth mentioning that ten of these cultivars are adapted to agro-ecological conditions of the main areas that produce cassava in Colombia (Northern Coast, Eastern Planes and Inter-Andean Valleys).

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