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CBN-V Video Archives - S7-04
Identification of the Genetic Variability in Cassava Clones Obtained
by different Propagation Methods in Cuba
Beovides Y.1,
C. González2,M.I.
Román1,
X. Xiqués2,
M. Florido3 ,
M. Lara3 ,V.
Medero1 ,S.
García1,L.
Díaz2,
M. Milián1,
H. Toledo1 and
D. Guerra1
1. Instituto de Investigaciones en Viandas Tropicales (INIVIT), Apdo.
6, Santo Domingo 53000, Villa Clara, Cuba.
inivit@ip.etecsa.cu
2. Facultad de Biología, Universidad de La Habana, Cuba.
3. Instituto Nacional de Ciencias Agrarias (INCA), La Habana, Cuba
Cassava (Manihot esculenta Cranz) is a major staple food for
almost a thousand million people throughout the world. A method for
its micropropagation through organogenesis and embryogenesis has
been refined in Cuba; however, there is a lack of surveys on the
characterization of these materials. Electrophoretic studies of
different isoenzymatic systems and total protein, as well as,
cytogenetic analysis are valid and rapid techniques for detecting
this variability. Cultivars ‘Señorita’, ‘CEMSA 74-725’ and ‘CMC-76’
were propagated by traditional methods, and through organogenesis
and somatic embryogenesis in order to determine possible genetic
variability due to these techniques. Forty four morphoagronomic
characteristics were evaluated, the chromosome number was determined
and the peroxidases, polyphenoloxidases, esterases, acid phosphatase,
dehydrogenase malate, carbonic anhydrase, dehydrogenase formiate
isoenzymatic systems and total protein were studied. The most
important morphoagronomic variables were defined and the diploid
condition of studied clones was confirmed with the presence of 2n=36
chromosomes for all materials. Some differences in the band patterns
for all variants were only found in esterases and peroxidases
systems and total proteins, however, the propagating methods used do
not produce a considerable variability in these clones. These
results which have been obtained for the first time, constitute an
important step for validating implemented methods, as well as, a
genetic characterization of the Cuban cassava germplasm in the
future.
2007©
Donald Danforth Plant Science Center All rights reserved.
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