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CBN-V Video Archives - S7-02
Light as a Crucial Factor for the Induction of Somatic Embryogenesis
in Latin American and African Cassava Cultivars
Akano A.O.1,2,
M.A. Fregene2, A.G.O. Dixon3, N.J. Taylor1
and C.M. Fauquet1
1. International Laboratory for
Tropical Agricultural Biotechnology (ILTAB)/Donald Danforth Plant
Science Center, 975 Warson Road St. Louis, MO USA
iltab@danforthcenter.org
2. International Center for Tropical Agriculture (CIAT), Cali
Colombia
3. International Institute of Tropical Agriculture, Ibadan Nigeria
Induction of embryogenic target tissues is a pre-requisite for
genetic engineering of cassava. Optimized conditions for generating
embryogenic structures in different cassava cultivars are required
if effective transformation systems are established for the crop.
Investigations were carried out to elucidate the optimum light
regime for embryogenesis in Latin American and African cassava
cultivars. Three light levels were tested:-dark, low light (0.42
µmol m-2 s-2) and high light (7.2 µmol m-2 s-2). Preteating in
vitro mother plants in different light regimes did not have a
significant influence on the induction of embryogenic tissues.
However, when young unopened leaf lobes were explanted onto
Murashige and Skoog basal media supplemented with 2% w/v sucrose and
50 M picloram, low light was found to be significantly superior to
both the dark and high light treatments, increasing the formation of
embryogenic structures by a factor of two in cv. TMS 60444.
Investigations in further cultivars continue. We conclude that light
levels can have important influence on the in vitro
morphogenic potential of cassava tissues and should, therefore, be
considered at all stages of embryogenic tissue induction and plant
regeneration.
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Donald Danforth Plant Science Center All rights reserved.
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