CBN-V Video Archives - S7-01
Cryopreservation of Cassava
(Manihot esculenta Crantz)

Adeniyi O.J.¹, M.O. Akoroda² and S.Y.C. Ng¹

1. International Institute of Tropical Agriculture, Ibadan, Nigeria
2. University of Ibadan, Ibadan, Nigeria sng@cgiar.org

        The purpose of this study was to develop and/or adopt the best cryopreservation protocol for African cassava germplasm. In this work, eleven protocols were involved. One protocol was carried out to check the tolerance of pregrowth meristems to DMSO (Dimethyl sulphoxide). The other ten were cryopreservation protocols and were employed to determine the best possible protocol in terms of recovery rate and genetic stability. Seven cultivars obtained from the International Institute of Tropical Agriculture, were used. These included TMS M80/00106, TMS 63397, TMS 60142, TMS 30001, TMS 91/02324, TME 1 and TME 2. The experimental layout was a split plot factorial design. At the end of the first protocol, out of the seven DMSO concentrations tested on pregrowth meristems of four genotypes, it was observed that 0.5% (v/v) DMSO was the concentration in which highest tolerance was found. Similarly, out of the 10 cryopreservation protocols carried out, a five hour dehydration using alginate-bead dehydration method was superior. It was also confirmed that modification introduced into the cryoprotection stage was reasonable. This was because 5% DMSO proved to perform best if used alone as cryoprotectant while 0.5% DMSO, combined with calcium alginate and high sucrose, proved equally good for cryopreservation of meristems and nodal cuttings.
 

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