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CBN-V Video Archives - S6-02
Identification of Molecular Markers for Characterization of
Brazilian Cassava Germplasm
Alves A.A.C.1
and D.D. dos Reis Filho2
1. Embrapa Cassava and Fruit Crops, Caixa Postal 007, 44.380-000,
Cruz das Almas, Bahia, Brazil
aalves@cnpmf.embrapa.br
2. Bahia Federal University / CNPq
The Brazilian cassava genebank at Embrapa (Brazilian Agricultural
Research Corporation), in Northeast Brazil, contains about 1700
accessions, which represent the largest genetic diversity within the
center of cassava origin. This collection has been fully
characterized by several morphological descriptors. Based on this
characterization and passport data, many groups of accessions,
having the same values for nine characters that are genetically
stable, were identified. Although these groups have a large
morphological similarity, it is not reliable to say that the
accessions within them are duplicates. To evaluate the usefulness of
molecular characterization in comparison to morphological
characterization, an analysis by RAPD using several primers were
performed on many pairs of genotypes with different degrees of
morphological similarity. The results showed that morphological
approach is not accurate to identify duplicates, since some
polymorphism were found between pairs of accessions morphologically
similar. The results emphasize the need to use molecular markers to
detect, more precisely, duplication within the cassava gene bank as
well as to validate the cassava core collection.
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Poster (Microsoft PowerPoint Document)
2007©
Donald Danforth Plant Science Center All rights reserved.
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