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CBN-V Video Archives - S5-13
Development of BAC Library Resources for Cassava
Tomkins
J.P.1, M. Fregene2, J. Tohme2 and
R.A. Wing1
1. Clemson University Genomics
Institute, Clemson, SC 29634 USA
2. CIAT Headquarters Apartado Aéreo 6713, Recta Cali-Palmira, Km 17,
Cali, Colombia m.fregene@cgiar.org
We have constructed a BAC library for cassava using the clone
MECU72. This clone carries resistance to the whitefly pest
Aleurothachellus socialis. The library contains 73,728 clones
stored in 192 384-well microtiter plates. A random sampling of BACs
indicated an average insert size of 102 kb. Based on a genome size
of 760 Mb, library coverage is approximately 10 haploid genome
equivalents. We are also now in the process of constructing a second
BAC library for cassava using the clone NGA2 which contains
resistance to the African cassava mosaic virus (ACMV). Data from BAC
library construction and characterization efforts will be discussed.
Both whitefly resistance and ACMV will be targets for map-based
cloning using the BAC libraries as tools.
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Donald Danforth Plant Science Center All rights reserved.
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