CBN-V Video Archives - S5-11
Protein Polymorphism in Starch Granules of Storage Roots of Cassava Clones with Different Genetic Backgrounds

Gomes A.P.G, T.L. Rocha and L.J.C.B. Carvalho

EMBRAPA-Genetic Resources and Biotechnology, CP 02372, CEP 70770-900 Sain Parque Rural, Brazilia, Brazil carvalho@cenargen.embrapa.br

        The proportion of amylose to amylopectine in starch granule has been proved to be dependent on the gene that codes for granule bound starch synthase (GBSS). Amylopectine structure has also been reported to be related to starch granule bound proteins of unknown enzymatic functions. Novel cassava clones with storage roots (SR) that accumulate different proportions of amylose:amylopectine have been isolated in our laboratory. In this communication we use these novel cassava clones to further understand the genetics of this starch diversity structure in the SR of cassava. Protein extracts from purified starch granules are being analyzed by SDS-PAGE, 2-DE gels, computer assisted (GELLAB-IIa software) analysis, MALDI-TOF protein characterization and protein sequencing. SDS-PAGE results indicated several single protein bands present in the CAS36 clones series that is not present in the commercial clones. Protein size varied from 100 to 40kDa molecular mass. The 2-DE gel analysis confirm this polymorphism and revealed the presence of proteins with pI varying from 4.6 to 6.8. Further protein characterization are underway to isolate the genes responsible for the starch structure diversity tested including starch from the cassava ancestor.

This research financed by the National Biotechnology Program (EMBRAPA)

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