CBN-V Video Archives - S5-09
Progress Towards Cloning the Single Dominant Gene Conferring Immunity to Cassava Mosaic Disease (CMD)

Fregene M.¹ H. Matsumura², A. Akano¹, A. Dixon³ and R. Terauchi²

1. Centro Internacional de Agricultura Tropical (CIAT), AA. 6713, Cali Colombia m.fregene@cgiar.org
2. Iwate Biotechnology Research Center (IBRC), Narita, Kitakami, Iwate 024-0003, Japan
3. International Institute of Tropical Agriculture (IITA), PMB 5320, Ibadan, Nigeria

        Cassava mosaic disease (CMD) is a viral disease of the important tropical staple cassava (Manihot esculenta). Preferred management is by host plant resistance. A novel source of resistance was recently identified in a Nigerian cassava land race that confers immunity to the disease. Serial analysis of gene expression (SAGE) was used to analyze the gene expression pattern in a F1 progeny derived from crossing TME3. the CMD resistant land race, and TMS30555, a susceptible improved line. A total of 12,786 transcripts were studied, divided into 5733 and 7053 tags from a bulk of resistant and susceptible genotypes respectively. Tag annotation was by PCR amplification using the tag sequence as sense primer and a cDNA library, constructed with mRNA from the resistant bulk, as template. Annotation of 18 transcripts revealed several genes expressed during systemic acquired resistance (SAR) in plants and other genes involved in cell entry, cell-to-cell and cytoplasm-to-nucleus virus trafficking. Genetic mapping was used to identify association between these genes and CMD resistance. Work is ongoing to test a candidate gene for their ability to confer CMD resistance in tobacco plants.

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