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| Consortium for Maize Genomics |
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Strategies: High-Cot
High Cot selected libraries produced by Jeff Bennetzen at Purdue University exploit the relatively low abundance of the gene sequences,
which are present in small number of copies in the genome.
- DNA from immature maize ears sheared to an average size of 1.0 to 2.0 kb
- Sheared DNA is denatured and then renatured to a C0t value of 800
- Unrenatured DNA is purified over a HAP column
- ssDNA converted to dsDNA
- dsDNA size selected to 1.2 to 2.0 kb
Go back to "Strategies"
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