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Jan Jaworski's Laboratory






Publications

Some reprints are available at the websites listed below.  Please note that subscriptions are needed to access some of these reprints.

Journal Articles

Tsegaye Y., Richardson C.G., Bravo J.E., Mulcahy B.J., Lynch D.V., Markham J.E., Jaworski J.G., Chen M., Cahoon E.B., Dunn T.M. (2007) Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J Biol Chem 282: 28195-28206.

Abstract available (full text with subscription)

King, A., Nam, J.-W., Han, J., Hilliard, J. and Jaworski, J, G.  (2007)  Cuticular wax biosynthesis in petunia petals: cloning and characterization of an alcohol-acyltransferase that synthesizes wax-esters.  Planta  226:381-394.

Abstract available (full text with subscription)

Markham, J. E. and Jaworski, J. G. (2007)  Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.  Rapid Commun Mass Spectrom. 21:1304-1314.

Zhang, Y., Li, S.-Z., Li, J., Pan, X., Cahoon, R. E., Jaworski, J. G., Wang, X., Jez, J. M., Chen, F., Yu, O.  (2006)  Using unnatural protein fusions to engineer resveratrol biosynthesis in yeast and mammalian cells.  J. Am. Chem. Soc. 128:13030-13031.

Abstract available (full text with subscription)

Markham , J.E., Li, J., Cahoon, E.B. and Jaworski, J.G.  (2006)  Separation and identification of major plant sphingolipid classes from leave.  J Biol Chem  281:22684-22694. 

Abstract available (full text with subscription)

Blacklock, B.J. and Jaworski, J.G.  (2006)  Substrate specificity of Arabidopsis 3-ketoacyl-CoA         synthases.  Biochem Biophys Res Commun  346:583-90. 

Paul, S., Gable, K., Beaudoin, F., Cahoon, E., Jaworski, J., Napier, J. A. and Dunn, T.M. (2006)  Members of the Arabidopsis FAE1-like 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharamyces cerevisiae.  J. Biol. Chem. 281: 9018-9029.  

Abstract available (full text with subscription)

Jaworski, J and Cahoon, EB (2003) Industrial oils from transgenic plants. Cur Opin Plant Biol 6: 178-184.  

Abstract available (full text with subscription)

Blacklock, BJ and Jaworski, JG (2002) Studies into factors contributing to substrate specificity of membrane-bound 3-Ketoacyl-CoA synthases. Eur J Biochem 269: 4789-4798.  

Abstract available (full text with subscription)

Ghanevati, M, and Jaworski, J. G. (2002) Engineering and mechanistic studies of Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS. Eur J Biochem 269: 3531-3539.  

Abstract available (full text with subscription)

Dehesh, K., Tai, H., Edwards, P., and Jaworski, J. G. (2001) Overexpression of 3-Ketoacyl-Acyl Carrier Protein Synthase IIIs (KAS III) reduces the rate of lipid synthesis. Plant Physiol 125: 1103-1114.  

Abstract available (full text with subscription)

Ghanevati, M., and Jaworski, J. G. (2001) Active-site residues of a plant membrane-bound fatty acid elongase b-ketotacyl-CoA synthase, FAE1 KCS. Biochim Biophys Acta 1530: 77-85.

White, JA, Todd, J, Newman, T, Focks, N Girke, T, Martínez de Ilárduya, O, Jaworski, JG, Ohlrogge, J, and Benning C (2000) A New Set of Arabidopsis ESTs from Developing Seeds: The Metabolic Pathway from Carbohydrates to Seed Oil. Plant Physiol 124: 1582-1594.  

Abstract available (full text with subscription)

Todd, J., Post-Beittenmiller, D. and Jaworski, J. G. (1999) KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana. Plant J 17:119-130.

Abstract available (full text with subscription)

Hlousek-Radojcic, A., Evenson, K. J., Jaworski, J. G. and D. Post-Beittenmiller. (1998) Fatty acid elongation is independent of acyl-CoA synthetase activities in leek and Brassica. Plant Physiol 116:251-258.  

Abstract available (full text with subscription)

Hlousek-Radojcic, A., Imai H., and Jaworski, J. G. (1995) Oleoyl-CoA is not an Immediate Substrate for Fatty Acid Elongation in Developing Seeds of Brassica napus. Plant J 8: 803-809.

Slabaugh, M. B., Tai, H., Jaworski, J., Knapp, S. J. (1995) CDNA clones encoding beta-ketoacyl-acyl carrier protein synthase III from Cuphea wrightii. Plant Physiol 108: 443-444.  

Full text available with subscription

Ohlrogge, J., Savage, L., Jaworski, J., Voelker, T., and Post-Beittenmiller, D. (1995) Alteration of acyl-ACP pools and acetyl-CoA carboxylase expression in E. coli by a plant medium chain acyl-ACP thioesterase. Arch Biochem Biophys 317: 185-190.

Kopka, J, Ohlrogge, J. B., and Jaworski, J. G. (1995) Analysis of in vivo Levels of Acyl-Thioesters with Gas Chromatography/Mass Spectrometry of the Butylamide-Derivative. Anal Biochem 224:51-60.

Tai, H., Post-Beittenmiller, D., and Jaworski, J. G. (1994) Cloning of a cDNA encoding 3-ketoacyl acyl carrier protein synthase III from Arabidopsis. Plant Physiol 106: 801-802.  

Full text available with subscription

Jaworski, J. G., Tai, H., Ohlrogge, J. B, and Post-Beittenmiller, D. (1994) The initial reactions of fatty acid biosynthesis in plants. Prog Lipid Res 33: 47-54.

Tai, H., and Jaworski, J. G. (1993) 3-Ketoacyl acyl carrier protein synthase III from spinach (Spinacia oleracea) is not similar to other condensing enzymes of fatty acid synthase. Plant Physiol 103: 1361-1367.  

Abstract available (full text with subscription)

Jaworski, J. G., Post-Beittenmiller, D., and Ohlrogge, J. B. (1993) Acetyl-Acyl Carrier Protein is not a Major Intermediate in Fatty Acid Biosynthesis in Spinach. Eur J Biochem 213: 981-987.

Clough, R. C., Matthis, A. L., Barnum, S. R., and Jaworski, J. G. (1992) Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach: a condensing enzyme utilizing acetyl-CoA to initiate fatty acid synthesis. J Biol Chem 267: 20992-20998.  

Abstract available (full text with subscription)

Post-Beittenmiller, D., Jaworski, J. G., and Ohlrogge, J. B. (1991) In vivo pools of free and acylated Acyl Carrier Proteins in spinach: evidence for sites of regulation of fatty acid biosynthesis. J Biol Chem 266: 1858-1865.  

Abstract available (full text with subscription)

Froehlich, J. F., Poorman, R., Reardon, E., Barnum, S. R., and Jaworski, J. G. (1990) Purification and characterization of Acyl Carrier Protein from two Cyanobacteria species. Eur J Biochem 193: 817-825.

Jaworski, J. G., Post-Beittenmiller, M. A., and Ohlrogge, J. B. (1989) Site-directed mutagenesis of spinach acyl carrier protein prosthetic group attachment site. Eur J Biochem 184:603-609.

Jaworski, J. G., Clough, R. C., and Barnum, S. R. (1989) A cerulenin insensitive short chain 3-ketoacyl-acyl carrier protein synthase in Spinacia oleracea leaves. Plant Physiol 90:41-44.

Clough, R.C., Barnum, S.R., and Jaworski, J.G. (1989) Synthesis of radiolabeled acetyl-coenzyme A from sodium acetate. Anal Biochem 172: 82-84.

Chen, H.-H., Wickrema, A., and Jaworski, J. G. (1988) Acyl-acyl carrier protein : lysomonogalactosyldiacylglycerol acyltransferase from the cyanobacterium, Anabaena variabilis. Biochimica Biophysica Acta 963:493-500.

Stapleton, S. R. and Jaworski, J. G. (1984) Characterization and purification of malonyl-CoA:acyl carrier protein transacylase from spinach and Anabaena variabilis. Biochimica Biophysica Acta 794: 240-248.

Stapleton, S. R. and Jaworski, J. G. (1984) Characterization of fatty acid biosynthesis in the cyanobacterium, Anabaena variabilis. Biochimica Biophysica Acta 794: 249-255.

Nabors, L. A., Morgan, M. S., Newman, D. W., and Jaworski, J. G. (1984) Preferential metabolism of linoleic acid by five-day old barley shoots. Lipids 19: 507-514.

Pierce, R. J., Wissing, T. E., Jaworski, J. G., Givens, R. N., and Megrey, B. A. (1980) Energy storage and utilization patterns of the gizzard shad in Acton Lake, Ohio. Transactions American Fisheries Societies 109: 611-616.

Place, M. A., Morgan, M. S., Rutkoski, A., Newman, D. W., and Jaworski, J. G. (1979) Fatty acid metabolism in senescing and regreening soybean cotyledons. Planta 147: 246-250.

Blume, D. E., Jaworski, J. G., and McClure, J. W. (1979) Uridinediphosphate-glucose:isovitexin 7-O-glucosyltransferase from barley protoplasts: subcellular localization. Planta 146: 199-202.

Dalgarn, D., Miller, P., Bricker, T., Speer, N., Jaworski, J. G., and Newman, D. W. (1979) Galactosyl transferase activity of chloroplast envelopes from senescent soybean cotyledons. Plant Science Letters 14: 1-6.

Rutkoski, A. and Jaworski, J. G. (1978) Fatty acid synthetase from chloroplasts of soybean cotyledons: ACP activation and CoA inhibition. Biochemistry and Biophysical Research Communications 84: 428-434.

Wetterau, J. R., Newman, D. W., and Jaworski, J. G. (1978) Quantitative changes of fatty acids in soybean cotyledons during senescence and regreening. Phytochemistry 17: 1265-1268.

Jaworski, J. G., Goldschmidt, E. E., and Stumpf, P. K. (1974) Fat metabolism in higher plants. LXIII. Palmitoyl-ACP elongating system from maturing Carthamus tinctorius. Archives of Biochemistry and Biophysics 163: 769-776.

Jacobson, B. S., Jaworski, J. G., and Stumpf, P. K. (1974) Fat metabolism in higher plants. LXII. Stearoyl-acyl carrier protein desaturase from spinach chloroplast. Plant Physiology 54: 484-486.

Jaworski, J. G., and Stumpf, P. K. (1974) Fat metabolism in higher plants. LX. Enzymatic preparation of E. coli stearoyl-ACP. Archives of Biochemistry and Biophysics 162: 166-173.

Jaworski, J. G., and Stumpf, P. K. (1974) Fat metabolism in higher plants. LIX. Properties of stearoyl-ACP desaturase from maturing Carthamus tinctorius. Archives of Biochemistry and Biophysics 162: 158-165.

Jaworski, J. G., and Kuc, J. (1974) Effects of ethrel and Ceratocystis fimbriata on the synthesis of fatty acids and 6-methoxymellein in carrot root. Plant Physiology 53: 331.

Jaworski, J. G., Kuc, J., and Williams, E. B. (1973) Effects of ethrel and Ceratocystis fimbriata on the accumulation of chlorogenic acid and 6-methoxymellein in carrot root. Phytopathology 63: 408-413.

Book Chapters and Invited Reviews

Somerville, C., Browse, J. Jaworski, J. G. and Ohlrogge, J. B. (2000) "Lipids" in Biochemistry and Molecular Biology of Plants. (B. B. Buchanan, W. Gruissem and R. L. Jones, eds.) Amer. Soc. Of Plant Physiologists, Rockville, MD, pp 456-527.

Ohlrogge, J., and Jaworski, J. (1997) Regulation of Plant Fatty Acid Biosynthesis. Ann. Rev. Plant Phys and Plant Mol Biol 48: 109-136.

Ohlrogge, J. B., Jaworski, J. G., and Post-Beittenmiller, D. (1993) "De Novo Fatty Acid Synthesis" in Lipid Metabolism in Plants. (Moore, T., ed) pp. 3-32. CRC Press Inc.

Jaworski, J. G. 1987. "Biosynthesis of Monoenoic and polyenoic fatty acids." in The Biochemistry of Plants (P. K. Stumpf and E. E. Conn, eds.) Volume 9, pp 159-174. Academic Press, Inc. N.Y.

Patents pending

"Fatty acid elongases" with Martha Post-Beittenmiller and James Todd

"Fatty acid elongase 3-Ketoacyl-CoA Synthase Polypeptides" with Brenda J. Blacklock

"Compositions and Methods for Omega-Hydroxylating Fatty Acids" with Jixiang Han


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