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Image spectroscopy-based separation of GFP and YFP-labeled
proteins.
Transient expression of a peroxisome-localized GFP and
Rab5-YFP (endosome marker). Panel in grayscale (left) is the
lambda stack (10.7 nm bandwidth spectral information
acquired using the Meta detector on a Zeiss LSM 510 confocal
microscope), color image (right) is the “unmixed” (deconvolved)
image, separating the signals:
blue = peroxisomes, red = “endosomes”, green =
autofluorescence. The markers were expressed separately in
these tobacco leaf epidermal cells. Image width = 110 µm. |
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ASTRACT for presentation at the 2002
Microscopy Society of America Meeting. (5.03MB) |
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Spectral imaging of
chlorophyll (green, in chloroplasts) and ER-localized-DSRed
in epidermal cells of tobacco leaf. Single confocal plane,
Zeiss Meta detector. Marker= 5µm |
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