| |
Animations: 3-D digital reconstructions made from confocal
microscopy. Click on the images to download an MPEG movie.
You may need a player to view. We recommend the Microsoft
Windows Media Player which can be downloaded for free at
http://windowsmedia.com/download/download.asp.
3-D distribution of TMV virus proteins in plant cells (tobacco
BY-2 protoplasts)
 |
This
three-dimensional reconstruction shows the distribution of
three TMV virus proteins within a single plant cell, imaged
16 hours after infection with the virus. The green-colored
bodies are comprised of TMV movement protein, which is
membrane bound (in part of the ER) and associated with
another virus protein shown in blue: TMV coat protein. The
enzyme responsible for TMV replication (virus replicase) is
shown in red. The 3-D animation (download by clicking this
image) made possible by this method of microscopy is
essential for analyzing the co-distribution of the three
virus proteins, which provides clues on how the virus
completes its infection cycle in the plant cell. (virus
proteins immunolabeled, marker = 5 µm). Click the
image to download the MPEG movie (4.5MB) |
|
CP-transgenic
 |
|
42W-transgenic
|
|
Click the image to download
the movie (2.7MB) |
Click the image to download
the movie (2.1MB) |
TMV replicase = red
TMV coat protein = blue
TMV movement protein = green |
|
 |
|
|
|
Click the image to download
the movie (2.05MB) |
Click the image to download
the movie (2.05MB) |
| |
|
Three dimensional distribution of TMV proteins in tobacco
BY2 protoplasts transgenic with wild-type coat protein (left
hand images) or a mutant coat protein (right hand image at
two magnifications). Plant cells expressing the coat protein
are resistant to virus infection and these images are used
to analyze the mechanism for this resistance. The cells on
the left show two distinct patterns of colocalization of
coat and movement protein. The coat protein is in pockets of
movement protein (upper left) or extensively colocalized
with movement protein (light blue in lower left image). The
mutant coat protein causes a significant reduction in the
amount of movement protein (in green, upper right image). At
higher magnification (lower right image) it is possible to
analyze co-distribution of the proteins in subcellular
regions (yellow color indicates colocalized replicase and
movement protein).
These confocal images are the work of Dr. Sebastian
Asurmendi, of Roger Beachy's lab in the DDPSC. |
|
