S6-02
Identification of Molecular Markers for Characterization of Brazilian
Cassava Germplasm
Alves
A.A.C.1 and D.D. dos Reis Filho2
1.
Embrapa Cassava and Fruit Crops, Caixa Postal 007, 44.380-000, Cruz das
Almas, Bahia, Brazil aalves@cnpmf.embrapa.br
2.
Bahia Federal University / CNPq
The
Brazilian cassava genebank at Embrapa (Brazilian Agricultural Research
Corporation), in Northeast Brazil, contains about 1700 accessions, which
represent the largest genetic diversity within the center of cassava
origin. This collection has
been fully characterized by several morphological descriptors.
Based on this characterization and passport data, many groups of
accessions, having the same values for nine characters that are
genetically stable, were identified.
Although these groups have a large morphological similarity, it
is not reliable to say that the accessions within them are duplicates.
To evaluate the usefulness of molecular characterization in
comparison to morphological characterization, an analysis by RAPD using
several primers were performed on many pairs of genotypes with different
degrees of morphological similarity.
The results showed that morphological approach is not accurate to
identify duplicates, since some polymorphism were found between pairs of
accessions morphologically similar.
The results emphasize the need to use molecular markers to
detect, more precisely, duplication within the cassava gene bank as well
as to validate the cassava core collection.
|
