S5-07
Towards the Identification of Cassava Storage Root
Protein Genes
de Souza C.R.B.,
E.R.P. de Almeida, L.J.C.B. Carvalho and E.S. Gander
EMBRAPA-Genetic
Resources and Biotechnology, CP 02372, CEP 70770-900 Sain Parque
Rural, Brazilia, Brazil carvalho@cenargen.embrapa.br
Cassava
(Manihot esculenta Crantz) is
a species of major importance to feed mankind in countries of Latin
America and Africa. The
storage root (SR) represents the major source of food with high
carbohydrate (85% DWt as starch) and low protein content (1-2% total
protein). Recently,
EMBRAPA-GRB has initiated a series of studies toward the identification
of new genes in cassava that are SR specific and developmentally
regulated by using a tissue compartmentalization model described in an
accompanying paper. In this
communication we report results of our studies in this system for
proteins extracted with phosphate saline buffer and fractionated in
alcohol. SDS-PAGE analysis, 2-DE analysis, protein sequencing and BLAST
sequence analysis were performed. Results
indicated that SDS-PAGE protein profiles are simple with proteins in the
molecular weight range of 14 to 100 kDa in three layers of SR cell zones
of the central cylinder. Three
proteins were partially sequenced with one showing identity with an
18kDa protein of the small heat shock protein family.
No sequence from NCBI databank matched with the two prolamin
protein sequences obtained. Isolation
and characterization of the genes of these unknown proteins are
currently underway.
This research financed by National Biotechnology Program (EMBRAPA)
and National Research Council (CNPq)
|
