S5-04
Analysis of Proteins Associated with Storage Root Formation in Cassava
using 2-D Gels
Cabral,
G.B. and L.J.C.B. Carvalho
EMBRAPA-Genetic
Resources and Biotechnology, CP 02372, CEP 70770-900 Sain Parque
Rural, Brazilia, Brazil carvalho@cenargen.embrapa.br
Two-dimensional
gel electrophoresis analysis was performed on adventitious and storage
roots of cassava (Manihot
esculenta Crantz). Non-swollen
adventitious roots and swollen storage root were obtained from the
accession WU104 grown in the greenhouse at the Department of Biology,
Washington University, St. Louis, MO-USA.
Saline buffer-soluble proteins were extracted, separated in a
high-resolution 2-D electrophoresis system and visualized using silver
staining gel procedure. Digital images were generate for further analysis.
Quantitative and qualitative protein spots analysis was performed
with a computer assisted image software system. Results revealed large
variation in the complexity of the gel protein profile between the two
root systems. About 90% of
the protein spots appeared in the pI range value of 4.0 to 6.5 and
between 14 to 80 Kda of molecular mass.
Detailed computer assisted analysis of this gel allowed us to
establish 5 distinct classes of protein based on spot quantification
that could be associated with swelling and non-swelling roots.
Variation in the complexity of protein pattern was related with
different type of root. Adventitious
roots showed a more simple profile related to primary growth, while the
storage roots displayed to be a more complex profile related to
secondary growth and starch accumulation.
This
research financed by the National Biotechnology Program (EMBRAPA), and
The Rockefeller Foundation (USA)
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