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Images:
NuStrandTL.mov (4.5 MB) - This is a time lapse movie of a cultured tobacco BY-2 cell (one frame every two seconds) made using DIC optics.
The central nucleus is surrounded by ER and cytoplasmic strands containing various organelles and ER can be seen growing from and
retracting to the nuclear region. In some cases the strands briefly touch the cell periphery and then retract. In other cases they stably bind the cell cortex.
strandDIC.mov (1.7 MB) - Another DIC movie of strand dynamics.
All of the other micrographs are also of tobacco BY-2 cells, in these the ER is fluorescent because the cells contain a ECFP-HDEL gene that labels the lumen of the ER.
This makes it possible to see the distribution of the ER, which consists of three easily distinguished locations. Most abundant is the cortical ER (CER) that is throughout
the cell cortex, anchored to the plasma membrane, and consists of interconnected tubular and cisternal elements (a reticulum). There is a similar array of
cisternal/tubular ER that surrounds the nucleus. There is also ER in the strands interconnecting the perinuclear ER with the CER.
3D files:
2Pslices.mov (4.3 MB) - a movie of an example of a stack of optical slices through a cell, data used in making 3D ER reconstructions (this is a stack of 250 sections through
a single cell, each separated by 0.3 µm).
CER - 3D reconstruction, an external view of the cell surface showing the cortical ER (CER) that lines the surface of the cell, immediately below the cell membrane, to
which it is anchored.
Accompanying movies: CERone.mov (11.8 MB) - same cell, general view of the CER,
CERflyby.mov (5.5 MB) - same cell, closer view of the CER, as a flyby.
strands1.jpg (105 KB), strands2.jpg (91 KB), strands3.jpg (104 KB), strands4.jpg (60 KB), - Various views of strands in the same cell as above (CER), showing the internal distribution of the ER, surrounding the nucleus and extending
from this to the cell periphery CER via strands of ER that anchor as connections to the CER.
Accompanying movies: Strands_internal.mov (2.7 MB) - same cell as the single images,
animation showing strands; strand_juncn.mov (8.0 MB) - closer view of strand junction
with the CER.
Live cell time lapse:
strandTL.mov (12.4 MB) - time lapse (one frame every 2 minutes) of a cutaway view of a tobacco BY-2 cell, 4D digital reconstruction. The strands connecting perinuclear
ER and CER are highly dynamic, moving along the CER. Nu_raft.mov (8.8 MB) - this time lapse also shows the strand dynamics, and especially shows the perinuclear ER
and movement of this as the strands themselves move.
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