These images are copyrighted to the Danforth Center and are not to be sold 
or published without the written permission of the Danforth Center.

We would like to acknowledge the support of the National Science Foundation as the 
source of funds to purchase the instruments used in making these images.

Light micrographs were made using a Zeiss LSM 5210 Meta NLO confocal/multiphoton 
system and are of living cells.

Electron micrographs were made using a Leo 912 AB transmission electron microscope.  
These thin section images are of samples that were physically fixed by ultra-rapid freezing 
(about 15 milliseconds) in a high pressure freezer, which prevents ice crystal formation 
which otherwise would destroy cell structure.  Compared to chemical fixation, ultra-rapid 
fixation produces the most life-like images currently possible in electron microscopy.